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regions included in a bicistronic vector enhances and stabilizes follistatin gene expressions in both transgeniccells and transgenic mice

Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 1, Pages 87-96 doi: 10.15302/J-FASE-2016087

Abstract: that the pMAR-CAG-FST-IRES-AcGFP1-polyA-MAR (pMAR-FST) vector had higher capacity to form monoclonal transgenicRemarkably, protein expression in transgenic cells with the pMAR-FST vector was significantly higherExogenous was expressed in all of the pMAR-FST transgenic mice at F , F and F .mice was significantly greater than in wild-type mice, with larger muscles in fore and hind limbs of transgenicmice. pMAR-FST transgenic mice were also found with more evenly distributed muscle bundles and thinner

Keywords： safety of transgenic bicistron gene transfer body transgenic mice muscle development

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Effects of the

Yi FANG,Xiangwei FU,Junjie LI,Ming DU,Baoyu JIA,Jinlong ZHANG,Xiaosheng ZHANG,Shien ZHU

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 4, Pages 314-320 doi: 10.15302/J-FASE-2014038

Abstract: This study was conducted to systematically assess the reproductive performance of transgenic ewes.In the transgenic founders (F ) and their positive offspring (F ), hematological and reproductive parametersand biochemical parameters determined from the blood samples did not differ significantly between the transgenicMoreover, the transgenic ewes showed reproductive traits similar to the wild-type ewes.Additionally, no significant differences were found between transgenic and wild-type ewes in the DNA

Keywords： TLR4 transgenic ewe safety assessment reproductive trait oocyte DNA methylation

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Advances of Transgenic Breeding on Rapeseed (Brassica napus)

Guan Chunyun

Strategic Study of CAE 2002, Volume 4, Issue 8, Pages 34-39

Abstract: It described advances of transgenic breeding of rapeseed (Brassica napus) in Oil Crops Institute, HunanThe. paper dealt with Bt-transgenic insect-resistant rapeseed line, transgenic sterile line, restorerline and their hybrid, transgenic high oleic acid content rapeseed line and transgenic high oil content

Keywords： rapeseed transgenic breeding

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Enlighenments for China from the Industrialization of the Transgenic Atlantic Salmon in the US

Hu Wei and Zhu Zuoyan

Strategic Study of CAE 2016, Volume 18, Issue 3, Pages 105-109 doi: 10.15302/J-SSCAE-2016.03.018

Abstract: the US Food and Drug Administration (FDA) approved the "all fish" growth hormone (gh) transgenicgenetically engineered livestock approved for human consumption and is considered as breakthrough of transgenicThe US FDA made this landmark decision after 20 years rigorous bio-safety assessment of the transgenicBoth the scientific research behind the creation of this transgenic product and the active participation

Keywords： transgenic Atlantic salmon industrialization enlightenments

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Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine

Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1, Pages 66-72 doi: 10.15302/J-FASE-2018229

Abstract: transfected into porcine fetal fibroblasts, G418 resistant transfectants were confirmed by PCR and transgenicThese results have paved the way for future production of marker-free transgenic resistant to PEDV and

Keywords： piglet diarrhea RNAi PEDV TGEV transgenic resistance breeding

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Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3, Pages 179-184 doi: 10.15302/J-FASE-2014020

Abstract: One potential method is to produce transgenic livestock that make modified milk containing high concentrationSubsequently, we injected the BAC construct into pronuclei of mouse fertilized embryos and generated transgenicanalysis showed that recombinant hBCHE (rhBCHE) was expressed efficiently in the mammary gland of the transgenicOur results pave the way for making transgenic livestock to produce large quantities of rhBCHE.

Keywords： recombinant human butyrylcholinesterase (rhBCHE) human lactoferrin bacterial artificial chromosome (hLF BAC) transgenic

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conventional mature B cells and compromised specific antibody response in bovine immunoglobulin μ heavy-chain transgenic

Min ZHANG,Xueqian CHENG,Dan CHU,Jingwen LIANG,Yi SUN,Li MA,Beilei XU,Min ZHENG,Meili WANG,Liming REN,Xiaoxiang HU,Qingyong MENG,Ran ZHANG,Ying GUO,Yunping DAI,Robert AITKEN,Ning LI,Yaofeng ZHAO

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 2, Pages 158-173 doi: 10.15302/J-FASE-2014015

Abstract: Bovine IgM was highly expressed in selected transgenic lines, and it largely inhibited rearrangementsAlthough the serum concentrations of endogenous IgM and IgG in the transgenic mice were significantlyThese transgenic mice also exhibited a lower frequency of unique complementarity determining region 3Compared to the WT mice, the transgenic mice had a significantly higher percentage of mouse IgM-expressingFinally, we showed that the transgenic mice were deficient in a specific antibody response to antigen

Keywords： bovine Ig μ heavy-chain transgenic mice B cell development allelic exclusion immune response Ig repertoire

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Urban design based on public safety-Discussion on safety-based urban design

Kaizhen CAI, Jianguo WANG

Frontiers of Structural and Civil Engineering 2009, Volume 3, Issue 2, Pages 219-227 doi: 10.1007/s11709-009-0023-4

Abstract: Because of public safety problems in construction of urban surroundings, this paper expounds the necessityof the study on public safety-based urban design in perspective of modern city.It brings forward the concept of safety-based urban design and attempts to explore the basic connotation

Keywords： urban public safety urban design safety-based urban design

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Study on the Priority of Food Safety Science and Technology in China

Wei Yimin,Wu Yongning,Zhou Naiyuan,Pan Jiarong

Strategic Study of CAE 2005, Volume 7, Issue 11, Pages 1-4

Abstract: its focus on food quality and safety, while developing economy.This paper, from the angle of developing national food safety strategy and strengthening food safetyis the scientific basis of food safety, and risk assessment is the key technology of food safety, itis suggested to be the first priority of food safety strategy., all responsible parties should bear the responsibility for food quality and safety.

Keywords： food safety science and technology of food safety strategy of food safety priority of food safety

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Probabilistic safety assessment of self-centering steel braced frame

Navid RAHGOZAR, Nima RAHGOZAR, Abdolreza S. MOGHADAM

Frontiers of Structural and Civil Engineering 2018, Volume 12, Issue 1, Pages 163-182 doi: 10.1007/s11709-017-0384-z

Abstract: states and investigates the seismic performance of self-centering braced frame using a Probabilistic SafetyMargin of safety, confidence level, and mean annual frequency of the self-centering archetypes for theirthat the design of low- and mid-rise self-centering archetypes could provide the adequate margin of safety

Keywords： self-centering steel braced frame mean annual frequency safety assessment confidence level margin ofsafety

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Study on Some Basic Problems of Safety Science

Lü Baohe

Strategic Study of CAE 2006, Volume 8, Issue 11, Pages 57-61

Abstract:

The scientific meanings of safety are analyzed.The concept of “zaishi” is introduced and the fact that it is just the research object of safetyrelationships are also discussed, based on which the necessity and feasibility of the construction of safetyscience are expatiated .The connotation of safety science and the basic approach of the constructionFinally，the hierarchy of system of safety science and technology is discussed

Keywords： safety “zaishi” safety science the construction of subject

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State of Practice of Construction Site Safety in the USA

Fei Dai,Yoojung Yoon,Hota V. GangaRao

Frontiers of Engineering Management 2016, Volume 3, Issue 3, Pages 275-282 doi: 10.15302/J-FEM-2016035

Abstract: Much safety improvements have been made after the USA Congress passed the Occupational Safety and HealthTo provide a holistic view of current construction safety practices and corresponding performance, thisIt then presents efforts led to prevent work-related accidents and injuries by the federal safety agenciesAdditionally, advantages and limitations of current practices in terms of construction safety have been

Keywords： workplace safety construction industry safety strategy accident prevention review work

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Analyzing construction safety through time series methods

Houchen CAO, Yang Miang GOH

Frontiers of Engineering Management 2019, Volume 6, Issue 2, Pages 262-274 doi: 10.1007/s42524-019-0015-6

Abstract: However, existing data sets have not been fully exploited in analyzing the safety factors of constructionThus, this work describes how temporal analysis techniques can be applied to improve the safety managementused herein was obtained from a large construction company that is based in Singapore and contains safety

Keywords： time series temporal construction safety leading indicators accident prevention forecasting

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Global challenges of food safety for China

Joseph J. JEN

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 3, Pages 291-293 doi: 10.15302/J-FASE-2017167

Abstract:

Ever since the Melamine event, China has faced challenges of food safety both domestically and globallymostly small and medium enterprises presents challenges to the government to monitor and implement food safetyDiscussions and potential solutions of the global challenges of the five pillars for the food safety

Keywords： food safety challenges food industry government academia media consumer

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A review of the safety and efficacy of current COVID-19 vaccines

Frontiers of Medicine 2022, Volume 16, Issue 1, Pages 39-55 doi: 10.1007/s11684-021-0893-y

Abstract: This study reviewed the efficacy/effectiveness, immunogenicity, and safety profile of the 12 most progressedOverall, most of the current vaccines have shown safety and efficacy/effectiveness during actual clinicalcoverage, strategies to address variants with immune escape capability, and surveillance of vaccine safety

Keywords： COVID-19 SARS-CoV-2 vaccine safety efficacy effectiveness immunogenicity

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Title Author Date Type Operation

regions included in a bicistronic vector enhances and stabilizes follistatin gene expressions in both transgeniccells and transgenic mice

Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI

Journal Article

Effects of the

Yi FANG,Xiangwei FU,Junjie LI,Ming DU,Baoyu JIA,Jinlong ZHANG,Xiaosheng ZHANG,Shien ZHU

Journal Article

Advances of Transgenic Breeding on Rapeseed (Brassica napus)

Guan Chunyun

Journal Article

Enlighenments for China from the Industrialization of the Transgenic Atlantic Salmon in the US

Hu Wei and Zhu Zuoyan

Journal Article

Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine

Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG

Journal Article

Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Journal Article

conventional mature B cells and compromised specific antibody response in bovine immunoglobulin μ heavy-chain transgenic

Min ZHANG,Xueqian CHENG,Dan CHU,Jingwen LIANG,Yi SUN,Li MA,Beilei XU,Min ZHENG,Meili WANG,Liming REN,Xiaoxiang HU,Qingyong MENG,Ran ZHANG,Ying GUO,Yunping DAI,Robert AITKEN,Ning LI,Yaofeng ZHAO

Journal Article

Urban design based on public safety-Discussion on safety-based urban design

Kaizhen CAI, Jianguo WANG

Journal Article

Study on the Priority of Food Safety Science and Technology in China

Wei Yimin,Wu Yongning,Zhou Naiyuan,Pan Jiarong

Journal Article

Probabilistic safety assessment of self-centering steel braced frame

Navid RAHGOZAR, Nima RAHGOZAR, Abdolreza S. MOGHADAM

Journal Article

Study on Some Basic Problems of Safety Science

Lü Baohe